determining dead space ventilator sar1000

CTB-SAP enhanced pLTF at 7 (CTB-SAP: 162☑8%, n=8 vs. pLTF was assessed in anesthetized, paralyzed and ventilated Sprague Dawley rats 7 and 28 days following bilateral intrapleural injections of: 1) CTB-SAP (25μg), or 2) unconjugated CTB and SAP (control). Here, we tested the hypothesis that phrenic motor neuron death is sufficient to enhance pLTF. This CTB-SAP model allows us to study the impact of respiratory motor neuron death on breathing without many complications attendant to ALS. Intrapleural injections of cholera toxin B fragment conjugated to saporin (CTB-SAP) selectively kill respiratory motor neurons and mimic motor neuron death observed in SOD1 G93A rats. We suggest that one trigger for this enhancement is motor neuron death itself.

In a rat model of ALS (SOD1 G93A), phrenic long-term facilitation (pLTF) following acute intermittent hypoxia (AIH) is enhanced greater than expected at disease end-stage but the mechanism is unknown. Amyotrophic lateral sclerosis (ALS) is a devastating disease leading to progressive motor neuron degeneration and death by ventilatory failure.

Determining dead space ventilator sar1000